Paint.NET

What's new in this version:

New Functionality:
- Enabled visualization of GC content as a color plot in Map view or base colors in Sequence view
- Added support for finding similar DNA sequences with mismatches or indels compared to the search query
- Added support for simulating the migration of supercoiled DNA molecules in agarose gels using TBE, TAE or SB buffer
- Added support for single-stranded DNA (ssDNA) sequences
- Enabled import of Sequencher project files (*.spf)
- Enabled "Undo" for edits in large sequences
- Added DNA ladders from DyneBio
- Added supercoiled MW markers from ELPIS BIOTECH and New England Biolabs
- Added BsmBI-v2 to the list of enzymes available from New England BioLabs
- Added fields for username and email address in the license registration dialog

Enhancements:
- Optimized storage of history for the following operations: Change Methylation, Change Transformation Strain, Set Origin, Flip, Insert/Delete/Replace, Linearize, Circularize
- Updated the supported protein feature types by adding new types (NonStdRes, Protein, Precursor, SecStr, Het, CDS, gene, misc_feature, unsure, variation), promoting Region subtypes to types, and adding new Site and Bond subtypes
- Added a note in Features view to indicate the presence of internal stop codons in a translated feature
- Enhanced the Preferences tools to allow more flexible default options for displaying ORFs
- Enabled carrying over feature qualifiers when creating a protein sequence using "Make Protein" or "Reverse Translate"
- Added the option to merge segments when using "Make Protein" on a multi-segment DNA feature
- Added a "Hide noncutters" check box in the Choose Enzymes dialog
- Enabled importing features from any supported file type when using "Import Features from a SnapGene File"
- Enabled more flexible batch conversion of chromatogram traces to other formats
- Improved search performance for large DNA sequences
- Configured the minimap to show both scrolled areas when two copies of Sequence view are visible
- Updated the format of the Preferences dialog, and added an "Agarose Gels" tab
- Added the option to designate a new collection as the Main Collection
- Enabled saving imported online sequences directly to a collection
- Added shortcuts in a collection Overview for navigating to the DNA Files, Protein Files, or Miscellaneous Files sections
- Enabled symbols to be entered in search queries when searching SnapGene Online Sequences
- Increased the size of the length indicator in the map label at the "Small" font size
- Consolidated all Fisher MW Markers for agarose gels in the Fisher Scientific set
- Configured the Nonredundant Commercial enzyme set to include similar enzymes that differ by methylation sensitivity
- Configured SnapGene to show the Launch dialog on macOS when the SnapGene icon in the Dock is clicked, if no SnapGene windows are open
- Changed the icon for enabling interrupted circle format for a linear DNA sequence in Map view
- Configured the "Export Map" and "Export History" options to be always enabled
- Improved the wording of various menu options and dialogs to provide greater clarity and consistency
- Added a message informing the user that files can be dragged into the list when using Align Multiple Sequences

Fixed:
- Fixed an issue that prevented cloning dialogs from allowing the use of hidden enzymes
- Corrected an issue in "Protein Search" whereby terminal stop codons were not included in the search query
- Ensured that edits in an alignment window do not cause inappropriate scrolling
- Corrected an issue that could prevent alignment of a high-quality sequence trace
- Ensured that the "Find" control in the Enzymes view chooser always shows a message to indicate if the enzyme is not in the chosen set
- Corrected an issue in which U's in overhangs resulting from linearizing were not preserved
- Configured "Select All" in the trace viewer context menu to actually select all
- Ensured that History view reflects changes after editing DNA ends
- Ensured that case changes in the "Find" entry field are preserved when the search is executed
- Ensured that imported RNA alignments are converted to DNA rather than protein alignments
- Corrected the license inactivity countdown to displays seconds rather than milliseconds
- Ensured that the "Accession Number:" label remains next to its entry field in the collection Search dialog
- Ensured that a "Sequence Name" search in the Protein Files area of a collection also searches the map labels and aliases
- Corrected an issue that resulted in alignment and collection windows not showing unsaved changes in the title bar on Windows and Linux
- Ensured more consistent sorting of enzymes in the Choose Enzymes dialog
- Streamlined the substitution matrix options presented when computing pairwise alignments
- Ensured that open alignments can be used as profiles when computing new alignments
- Corrected an issue that could result in the "Kind" column disappearing when viewing a collection
- Ensured that crisp screenshots are shown when detecting updates
- Improved import of the full publication date from PubMed
- Made various stability fixes